Urine cytology examination aim to identify cells in a urine specimen from the urinary system by examining them under a microscope.

Lab report (1)

Cytology of Urine
Sedimentation and smearing

Student name
Academic number

 
Objective:
Urine cytology examination aim to identify cells in a urine specimen from the urinary system by examining them under a microscope.

Materials & Procedure:
Materials:
ᴥ Disposable dropper.
ᴥ Clean centrifuge tube.
ᴥ Clean microscope slide.
ᴥ Coverslip.
ᴥ Centrifuge.
ᴥ Light microscope

Procedure:
The steps are as follows:
1. In the beginning the urine sample was mixed then 5 ml was placed in a clean centrifuge tube.
2. Next urine was centrifuged at 1500 rpm for five minutes.
After centrifugation was completed, sediment may be visible at the bottom of the tube
3. Supernatant was discarded, carefully avoiding disruption of the material at the bottom, leaving 2 to 3 drops of supernatant to remix with the sediment.
4. Gently the tube was flicked with a finger to reconstitute the sediment with the remaining urine.
5. Then sediment was transferred to a clean microscope slide with a disposable dropper.
6. Coverslip was placed over the sample, finally microscopic examination of the sediment is performed on low power (10×), to evaluate the quantity of material present and the quality of the sample preparation.
High-power examination to evaluate cell morphology and number and identify casts and crystals. Each of these elements may be counted by averaging the number of elements in 10 fields.
Result:
After the urine specimen was examined under the microscope different elements were identified as represented below table:

White and Red blood cells

Epithelial Cells

Casts

Cast

Candida albicans

Transitional cell