Practice to Collect sample from Buccal, smear preparation and Squamous cell staining with Papanicolaou (Pap) stain.

Lab report ( 4 )
Buccal Smear Preparation and Squamous cell Staining

Tahani Al-Thagafi Student name
44480480 Academic number

 
Objective:
Practice to Collect sample from Buccal, smear preparation and Squamous cell staining with Papanicolaou (Pap) stain.

Material:
ᴥ Microscopic glass slide.
ᴥ Wooden stick or spatula.
Glass coverslip. ᴥ
. Alcohol 95% , 90% and 70% ᴥ
ᴥ Papanicolaou (Pap) stain .

Collection:
Gently mouth was scrapped with the wooden stick for 5-10 seconds.
Then, smear in wooden stick was speared over the glass slide for 2 – 3 seconds.

Procedure :
First slide was fixed in 95% ethyl alcohol fixative immediately for 15 minutes. Then it was treated with 70% alcohol for 2 minutes. After that, it was treated with tap water for 2 minutes. It was stained in Harr’s hematoxylin for 4 minutes. Rinsed in tap water briefly. Next dehydrated in absolute alcohol two change. it was stained in orange G for 2-3 minutes. Rinsed in absolute alcohol two change. Stained in E.A. 50 3 -5 minutes. Rinsed in 70%, 90% alcohol, and absolute alcohol two dips each of one. follow by cleared in xylene and mounted by DPX. At the end Examined under a light microscope.

Result:
After being examined under a microscope was observed squamous epithelial cells from the oral mucosa.

Squamous epithelial cell under 10x
Stained by Papanicolaou

Squamous epithelial cell under 40x
Stained by Papanicolaou