Practice to Collect sample from Buccal, smear preparation, and Squamous cell staining with hematoxylin and eosin (H&E) .

Lab report (3)

Buccal Smear Preparation and Squamous cell Staining

Tahani Al-Thagafi Student name
44480480 Academic number

Objective:
Practice to Collect sample from Buccal, smear preparation, and Squamous cell staining with hematoxylin and eosin (H&E) .

Material:
ᴥ Microscopic glass slide.
ᴥ Wooden stick or spatula.
Glass coverslip. ᴥ
95% , 90% and 70% alcohol . ᴥ
H & E stain.ᴥ

Collection:
Gently mouth was scrapped with the wooden stick for 5-10 seconds.
Then, smear in wooden stick was speared over the glass slide for 2 – 3 seconds.

Procedure:
First slide was fixed in 95% ethyl alcohol fixative immediately for 15 minutes. Then it was treated with 70% alcohol for 2 minutes. After that it was treated by tap water for 2 minutes. It was stained in Harr’s hematoxylin for 5 – 8 minutes. Rinsed in tap water briefly. Next Stained with eosin for 3 – 5 mins. Dehydrated in descending alcohol start with 70% , 90% , 95% then dipped in xylene and mounted with DPX. Finally slide was examined under microscope.

Result:
After being examined under the microscope was observed squamous epithelial cells from the oral mucosa.