Performing urine smear was stained with Diff-Quik stain and examined under different power to identify various cellular elements in the urine sample.

Lab report (2)

Preparation of urine for cytology

Tahani Al-Thagafi Student name
44480480 Academic number

 
Objective:
Performing urine smear was stained with Diff-Quik stain and examined under different power to identify various cellular elements in the urine sample.

Materials:
ᴥ Disposable dropper.
ᴥ Clean centrifuge tube.
ᴥ Centrifuge.
ᴥ Clean microscope slide.
ᴥ Spreader slide.
ᴥ Light microscope.
procedure:
In the first step centrifuge tube was filled with well-mixed, fresh urine taken from the bottom of the sample container.
The sample was centrifuged (and a balance tube)
After centrifugation, a concentrated pellet of formed elements should be visible at the bottom of the tube.
Then gently the supernatant was removed, and a small amount of urine was leaved to resuspend the pellet.
The bottom of the tube was Lightly flicked multiple times with a finger to gently resuspend the formed elements.
Next, the slide was labeled appropriately
A new pipette was used to add a drop of sample on a glass slide, similar to preparing a blood film.
} Clean glass spreader slide was placed on the labeled slide, at approximately 30°–40°, in front of the drop of urine.
The Spreader slide was backed into the drop allowing the material to spread along the edge of the spreader slide.
It was moved toward the end of the specimen slide, keeping the two in contact with each other.
In the middle of the slide, abruptly the urine sample was stopped spread, and lift the spreader slide straight up to form a line of material.

Air dry thoroughly and then the slide was stained using Romanowsky cytology stain

Stain component and method:
The Diff-Quik stain consists of
a fixative agent (methanol, blue).
Solution I (eosinophilic, orange) .
Solution II (basophilic, blue).
Generally, the slide was dipped sequentially into each solution 5 – 6 times and allowed excess to drained after each dip.
Then the slide was rinsed in distilled water
The slide was blotted or allowed to dry in air.
examined at low power to identify structures and then under high power. Finally

Results: After the smear was examined under a microscope were seen various cellular elements were as below shown:

Red &White blood cells Crystal

Hyphae urothelial cell Cluster of